陰謀分析_ワク05

スパイクたんぱく質はDNA修復とV(D)J遺伝子再構成を障害する(in Vitro)

(V(D)J遺伝子再構成（VDJいでんしさいこうせい、英：V(D)J recombinationまたはsomatic recombination）は、免疫システム内の免疫グロブリン（Ig）・TCR（T細胞受容体）生成の初期ステージにおける遺伝子再構成の仕組み。初期のリンパ組織（骨髄ではB細胞、胸腺ではT細胞）で起こる。V(D)J遺伝子再構成は、骨髄や胸腺でのリンパ球の遺伝子断片（V、D、J）のランダムな組み合わせである。抗体やTCRをコードするDNAは多数の断片として染色体の上に並んでいる。これらのDNA断片はリンパ球の分化の過程で連結され、完全なDNAとなる。いろんな遺伝子をランダムに選べるので、いろんなタンパク質をつくり、いろんな抗原（バクテリア、ウイルス、寄生菌、腫瘍、花粉など）に対抗することができる。by Wilipedia)

SARS–CoV–2  Spike  Impairs  DNA  Damage  Repair and Inhibits V(D)J  Recombination In Vitro, Received:  20  August  2021

Severe acute respiratory syndrome coronavirus 2 (SARS–CoV–2) has led to the coron- avirus disease 2019 (COVID–19) pandemic, severely affecting public health and the global economy. Adaptive immunity plays a crucial role in fighting against SARS–CoV–2 infection and directly in- fluences the clinical outcomes of patients. Clinical studies have indicated that patients with severe COVID–19 exhibit delayed and weak adaptive immune responses; however, the mechanism by which SARS–CoV–2 impedes adaptive immunity remains unclear. Here, by using an in vitro cell line, we report that the SARS–CoV–2 spike protein significantly inhibits DNA damage repair, which is required for effective V(D)J recombination in adaptive immunity. Mechanistically, we found that the spike protein localizes in the nucleus and inhibits DNA damage repair by impeding key DNA repair protein BRCA1 and 53BP1 recruitment to the damage site. Our findings reveal a potential molecular mechanism by which the spike protein might impede adaptive immunity and underscore the potential side effects of full-length spike-based vaccines.